An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

Malinova A, Cvackova Z, Mateju D, Horejsi Z, Abeza C, Vandermoere F, Bertrand E, Stanek D, Verheggen C

Splicing is catalyzed by the spliceosome, a complex of five major small nuclear ribonucleoprotein particles (snRNPs). The pre-mRNA splicing factor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a central module of the spliceosome. Using quantitative proteomics, we identified assembly intermediates containing PRPF8, EFTUD2, and SNRNP200 in association with the HSP90/R2TP ... [more]

J. Cell Biol. Dec. 05, 2016; 216(6);1579-1596 [Pubmed: 28515276]

Throughput

High Throughput

Additional Notes

Eftud2-DADE

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PARP1 EFTUD2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Krastev DB (2022)	High	-	BioGRID	3404594

Curated By

BioGRID

Interaction Summary

EFTUD2

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

PARP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD+ ADP-ribosyltransferase activity [IDA, TAS]enzyme binding [IPI]identical protein binding [IPI]poly(A) RNA binding [IDA]protein N-terminus binding [IPI]protein binding [IPI]transcription factor binding [IPI]