BAIT

EFTUD2

MFDGA, MFDM, SNRNP116, Snrp116, Snu114, U5-116KD
elongation factor Tu GTP binding domain containing 2
GO Process (5)
GO Function (2)
GO Component (7)
Homo sapiens
PREY

VCP

ALS14, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97
valosin containing protein
GO Process (18)
GO Function (8)
GO Component (13)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

Malinova A, Cvackova Z, Mateju D, Horejsi Z, Abeza C, Vandermoere F, Bertrand E, Stanek D, Verheggen C

Splicing is catalyzed by the spliceosome, a complex of five major small nuclear ribonucleoprotein particles (snRNPs). The pre-mRNA splicing factor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a central module of the spliceosome. Using quantitative proteomics, we identified assembly intermediates containing PRPF8, EFTUD2, and SNRNP200 in association with the HSP90/R2TP ... [more]

J. Cell Biol. Dec. 05, 2016; 216(6);1579-1596 [Pubmed: 28515276]

Throughput

  • High Throughput

Additional Notes

  • Eftud2-DADE

Curated By

  • BioGRID