BAIT

CDC6

mis10, pol3, pold, SPBC336.04
DNA polymerase delta catalytic subunit Cdc6
Schizosaccharomyces pombe (972h)
PREY

CDC1

mis1, SPAC27E2.05
DNA polymerase delta small subunit Cdc1
GO Process (4)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit.

Sanchez Garcia J, Ciufo LF, Yang X, Kearsey SE, MacNeill SA

DNA polymerase delta (Pol delta) plays a central role in eukaryotic chromosomal DNA replication, repair and recombination. In fission yeast, Pol delta is a tetrameric enzyme, comprising the catalytic subunit Pol3 and three smaller subunits, Cdc1, Cdc27 and Cdm1. Previous studies have demonstrated a direct interaction between Pol3 and Cdc1, the B-subunit of the complex. Here it is shown that ... [more]

Nucleic Acids Res. Jun. 03, 2004; 32(10);3005-16 [Pubmed: 15173383]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDC1 CDC6
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-PomBase
882464
CDC1 CDC6
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
CDC1 CDC6
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-PomBase
-
CDC1 CDC6
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-PomBase
882465
CDC1 CDC6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID