GPA2
Gene Ontology Biological Process
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [IPI]
- adenylate cyclase-activating glucose-activated G-protein coupled receptor signaling pathway [IMP]
- detection of glucose [IMP]
- negative regulation of G2/M transition of mitotic cell cycle [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]
- positive regulation of cAMP biosynthetic process [IMP]
- positive regulation of cAMP-dependent protein kinase activity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CYR1
Gene Ontology Biological Process
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [NAS]
- cAMP biosynthetic process [IGI, IMP]
- glucose mediated signaling pathway [IC]
- negative regulation of induction of conjugation with cellular fusion [IMP]
- negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]
- positive regulation of cAMP-mediated signaling [EXP]
- positive regulation of protein import into nucleus [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Direct activation of fission yeast adenylate cyclase by the Gpa2 Galpha of the glucose signaling pathway.
G protein-mediated signaling is implicated in yeast and fungal cAMP pathways. By two-hybrid screens and pull-down experiments, we show that the fission yeast Gpa2 Galpha binds an N-terminal domain of adenylate cyclase, comprising a moderately conserved sequence within a region otherwise poorly related to other fungal adenylate cyclases. Overexpressing this domain in yeast perturbs cAMP signaling, which is restored by ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GPA2 CYR1 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | PomBase | 883192 | |
| CYR1 GPA2 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | PomBase | - | |
| CYR1 GPA2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| CYR1 GPA2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | PomBase | - | |
| GPA2 CYR1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID