CYR1
Gene Ontology Biological Process
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [NAS]
- cAMP biosynthetic process [IGI, IMP]
- glucose mediated signaling pathway [IC]
- negative regulation of induction of conjugation with cellular fusion [IMP]
- negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]
- positive regulation of cAMP-mediated signaling [EXP]
- positive regulation of protein import into nucleus [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GPA2
Gene Ontology Biological Process
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [IPI]
- adenylate cyclase-activating glucose-activated G-protein coupled receptor signaling pathway [IMP]
- detection of glucose [IMP]
- negative regulation of G2/M transition of mitotic cell cycle [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]
- positive regulation of cAMP biosynthetic process [IMP]
- positive regulation of cAMP-dependent protein kinase activity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Direct activation of fission yeast adenylyl cyclase by heterotrimeric G protein gpa2.
Genetic studies on Schizosaccharomyces pombe adenylyl cyclase (cyr1) have shown that its activity is positively regulated by a heterotrimetric G protein a subunit gpa2 and that the resulting increase in intracellular cAMP concentration causes inhibition of sexual development including mating and meiosis. However, molecular mechanism underlying this gpa2-dependent regulation of cyr1 remains to be clarified. Here, we show that gpa2 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GPA2 CYR1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| GPA2 CYR1 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | PomBase | 883192 | |
| CYR1 GPA2 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | PomBase | - | |
| CYR1 GPA2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | PomBase | - | |
| GPA2 CYR1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID