BAIT

SAE2

COM1, ssDNA endodeoxyribonuclease SAE2, L000002892, YGL175C
Endonuclease required for telomere elongation; also required for telomeric 5' C-rich strand resection; involved in processing hairpin DNA structures with MRX complex; involved in double-strand break repair; required for normal resistance to DNA-damaging agents; exists in form of inactive oligomers that are transiently released into smaller active units by a series of phosphorylations; DNA damage triggers removal of Sae2p ensuring that active Sae2p is present only transiently
Saccharomyces cerevisiae (S288c)
PREY

SAE2

COM1, ssDNA endodeoxyribonuclease SAE2, L000002892, YGL175C
Endonuclease required for telomere elongation; also required for telomeric 5' C-rich strand resection; involved in processing hairpin DNA structures with MRX complex; involved in double-strand break repair; required for normal resistance to DNA-damaging agents; exists in form of inactive oligomers that are transiently released into smaller active units by a series of phosphorylations; DNA damage triggers removal of Sae2p ensuring that active Sae2p is present only transiently
Saccharomyces cerevisiae (S288c)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Regulatory control of DNA end resection by Sae2 phosphorylation.

Cannavo E, Johnson D, Andres SN, Kissling VM, Reinert JK, Garcia V, Erie DA, Hess D, Thomae NH, Enchev RI, Peter M, Williams RS, Neale MJ, Cejka P

DNA end resection plays a critical function in DNA double-strand break repair pathway choice. Resected DNA ends are refractory to end-joining mechanisms and are instead channeled to homology-directed repair. Using biochemical, genetic, and imaging methods, we show that phosphorylation of Saccharomyces cerevisiae Sae2 controls its capacity to promote the Mre11-Rad50-Xrs2 (MRX) nuclease to initiate resection of blocked DNA ends by ... [more]

Nat Commun Oct. 01, 2018; 9(1);4016 [Pubmed: 30275497]

Throughput

  • Low Throughput

Additional Notes

  • in vitro oligomerization

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SAE2 SAE2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
SAE2 SAE2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
1239745
SAE2 SAE2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
268410
SAE2 SAE2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID