BAIT

SLM2

LIT1, YNL047C
Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

MSS4

1-phosphatidylinositol-4-phosphate 5-kinase, L000001203, YDR208W
Phosphatidylinositol-4-phosphate 5-kinase; involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
Kinome Project (Sc)
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton.

Audhya A, Loewith R, Parsons AB, Gao L, Tabuchi M, Zhou H, Boone C, Hall MN, Emr SD

To further understand the roles played by the essential phosphoinositide PI4,5P(2), we have used a synthetic lethal analysis, which systematically combined the mss4(ts) mutation, partially defective in PI4P 5-kinase activity, with each of approximately 4700 deletion mutations. This genomic screening technique uncovered numerous new candidate effectors and regulators of PI4,5P(2) in yeast. In particular, we identified Slm1 (Yil105c), a previously ... [more]

EMBO J. Oct. 01, 2004; 23(19);3747-57 [Pubmed: 15372071]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: actin cytoskeleton morphology (APO:0000120)
  • phenotype: heat sensitivity (APO:0000147)

Additional Notes

  • slm1 slm2 background

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLM2 MSS4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4525BioGRID
2064172

Curated By

  • BioGRID