BAIT

PRPF40A

2810012K09Rik, FBP11, Fnbp11, Fnbp3, RP23-282A22.2
PRP40 pre-mRNA processing factor 40 homolog A (yeast)
GO Process (4)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

WASF3

Scar3, Wave3
WAS protein family, member 3
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation.

Mizutani K, Suetsugu S, Takenawa T

WASP family proteins are involved in cortical actin cytoskeleton reorganization. Neural Wiskott-Aldrich syndrome protein (N-WASP), a ubiquitously expressed WASP homologous protein, directly binds with Cdc42, activating Arp2/3 complex. In this study, we show that N-WASP-dependent microspike formation is inhibited by formin binding protein 11 (FBP11). Endogenous FBP11 localizes with nuclear-speckles, and co-localization of N-WASP and FBP11 was observed when they ... [more]

Biochem. Biophys. Res. Commun. Jan. 16, 2004; 313(3);468-74 [Pubmed: 14697212]

Throughput

  • Low Throughput

Curated By

  • BioGRID