An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The low density lipoprotein receptor-related protein LRP is regulated by membrane type-1 matrix metalloproteinase (MT1-MMP) proteolysis in malignant cells.

Rozanov DV, Hahn-Dantona E, Strickland DK, Strongin AY

We demonstrate that the presentation of LRP and the subsequent uptake of its ligands by malignant cells are both strongly regulated by MT1-MMP. Because LRP is essential for the clearance of multiple ligands, these findings have important implications for many pathophysiological processes including the pericellular proteolysis in neoplastic cells as well as the fate of the soluble matrix-degrading proteases such ... [more]

J. Biol. Chem. Feb. 06, 2004; 279(6);4260-8 [Pubmed: 14645246]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

LRP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

apolipoprotein binding [IDA, IPI]calcium ion binding [ISS]lipoprotein particle receptor binding [IC]lipoprotein transporter activity [NAS]poly(A) RNA binding [IDA]protein binding [IPI]protein complex binding [IDA]receptor activity [TAS]

Gene Ontology Cellular Component

MMP14