RSP5
Gene Ontology Biological Process
- cellular response to UV [IMP]
- chromatin assembly or disassembly [IMP]
- late endosome to vacuole transport via multivesicular body sorting pathway [IMP, IPI]
- mitochondrion organization [IGI, IMP]
- positive regulation of endocytosis [IMP]
- positive regulation of fatty acid biosynthetic process [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of receptor-mediated endocytosis [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IPI]
- protein autoubiquitination [IGI]
- protein monoubiquitination [IDA, IGI, IMP]
- protein polyubiquitination [IDA, IMP]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of actin cytoskeleton organization [IGI]
- regulation of dolichol biosynthetic process [IGI, IMP]
- regulation of ergosterol biosynthetic process [IGI, IMP]
- regulation of initiation of mating projection growth [IMP]
- regulation of mRNA export from nucleus [IMP, IPI]
- regulation of multivesicular body size [IMP]
- regulation of nitrogen utilization [IGI]
- regulation of phosphate metabolic process [IGI]
- regulation of protein localization [IMP, IPI]
- regulation of rRNA processing [IMP]
- regulation of ribosomal large subunit export from nucleus [IMP]
- regulation of tRNA export from nucleus [IMP]
- regulation of tRNA processing [IMP]
- regulation of ubiquinone biosynthetic process [IGI, IMP]
- response to drug [IMP, IPI]
- ribophagy [IGI]
- ubiquitin-dependent endocytosis [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SLA2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Functional dissection of a HECT ubiquitin E3 ligase.
Ubiquitination is one of the most prevalent protein post-translational modifications in eukaryotes, and its malfunction is associated with a variety of human diseases. Despite the significance of this process, the molecular mechanisms that govern the regulation of ubiquitination remain largely unknown. Here we used a combination of yeast proteome chip assays, genetic screening, and in vitro/in vivo biochemical analyses to ... [more]
Throughput
- High Throughput|Low Throughput
Additional Notes
- in vitro HTP screen
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RSP5 SLA2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SLA2 RSP5 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3463 | BioGRID | 2066131 | |
| RSP5 SLA2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 157332 | |
| RSP5 SLA2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 2533294 |
Curated By
- BioGRID