STK39
Gene Ontology Biological Process
- cellular hypotonic response [IC]
- intracellular signal transduction [IDA]
- negative regulation of potassium ion transmembrane transport [IDA]
- negative regulation of potassium ion transmembrane transporter activity [IDA]
- negative regulation of rubidium ion transmembrane transporter activity [IDA]
- negative regulation of rubidium ion transport [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- protein phosphorylation [NAS]
- response to stress [NAS]
- signal transduction by phosphorylation [IDA]
Gene Ontology Molecular Function
AATK
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Characterization of the interaction of the stress kinase SPAK with the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a scaffolding role of the kinase.
Activity of heterologously expressed NKCC1 was analyzed under basal and activated conditions in the presence and absence of binding of Ste20-related proline-alanine-rich kinase (SPAK). Mutant NKCC1 that lacks the ability to bind to this kinase showed K+ transport function identical to wild-type NKCC1. Thus, preventing the binding of the kinase to the cotransporter does not affect cotransporter function. In contrast, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AATK STK39 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 0 | BioGRID | 3509930 |
Curated By
- BioGRID