BAIT
STK39
DCHT, PASK, SPAK
serine threonine kinase 39
GO Process (11)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- cellular hypotonic response [IC]
- intracellular signal transduction [IDA]
- negative regulation of potassium ion transmembrane transport [IDA]
- negative regulation of potassium ion transmembrane transporter activity [IDA]
- negative regulation of rubidium ion transmembrane transporter activity [IDA]
- negative regulation of rubidium ion transport [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- protein phosphorylation [NAS]
- response to stress [NAS]
- signal transduction by phosphorylation [IDA]
Gene Ontology Molecular Function
Homo sapiens
PREY
OTOF
AUNB1, DFNB6, DFNB9, FER1L2, NSRD9
otoferlin
GO Process (2)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Characterization of the interaction of the stress kinase SPAK with the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a scaffolding role of the kinase.
Activity of heterologously expressed NKCC1 was analyzed under basal and activated conditions in the presence and absence of binding of Ste20-related proline-alanine-rich kinase (SPAK). Mutant NKCC1 that lacks the ability to bind to this kinase showed K+ transport function identical to wild-type NKCC1. Thus, preventing the binding of the kinase to the cotransporter does not affect cotransporter function. In contrast, ... [more]
J. Biol. Chem. Dec. 26, 2003; 278(52);52848-56 [Pubmed: 14563843]
Throughput
- Low Throughput
Curated By
- BioGRID