BAIT
STK39
DCHT, PASK, SPAK
serine threonine kinase 39
GO Process (11)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- cellular hypotonic response [IC]
- intracellular signal transduction [IDA]
- negative regulation of potassium ion transmembrane transport [IDA]
- negative regulation of potassium ion transmembrane transporter activity [IDA]
- negative regulation of rubidium ion transmembrane transporter activity [IDA]
- negative regulation of rubidium ion transport [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- protein phosphorylation [NAS]
- response to stress [NAS]
- signal transduction by phosphorylation [IDA]
Gene Ontology Molecular Function
Homo sapiens
PREY
HSPH1
HSP105, HSP105A, HSP105B, NY-CO-25, RP11-173P16.1
heat shock 105kDa/110kDa protein 1
GO Process (3)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Characterization of the interaction of the stress kinase SPAK with the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a scaffolding role of the kinase.
Activity of heterologously expressed NKCC1 was analyzed under basal and activated conditions in the presence and absence of binding of Ste20-related proline-alanine-rich kinase (SPAK). Mutant NKCC1 that lacks the ability to bind to this kinase showed K+ transport function identical to wild-type NKCC1. Thus, preventing the binding of the kinase to the cotransporter does not affect cotransporter function. In contrast, ... [more]
J. Biol. Chem. Dec. 26, 2003; 278(52);52848-56 [Pubmed: 14563843]
Throughput
- Low Throughput
Curated By
- BioGRID