KDM1A
Gene Ontology Biological Process
- blood coagulation [TAS]
- histone H3-K4 demethylation [IDA]
- histone H3-K9 demethylation [IDA]
- muscle cell development [ISS]
- negative regulation of DNA binding [IC]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- negative regulation of protein binding [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein demethylation [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- MRF binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- demethylase activity [IMP]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA]
- histone demethylase activity [IDA]
- histone demethylase activity (H3-K4 specific) [IDA]
- histone demethylase activity (H3-K9 specific) [IDA]
- histone demethylase activity (H3-dimethyl-K4 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP]
- oxidoreductase activity [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- transcription factor binding [IDA]
- transcription regulatory region DNA binding [ISS]
- MRF binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- demethylase activity [IMP]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA]
- histone demethylase activity [IDA]
- histone demethylase activity (H3-K4 specific) [IDA]
- histone demethylase activity (H3-K9 specific) [IDA]
- histone demethylase activity (H3-dimethyl-K4 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP]
- oxidoreductase activity [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- transcription factor binding [IDA]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
MBD2
Gene Ontology Biological Process
Gene Ontology Molecular Function- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- methyl-CpG binding [IDA, NAS]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- satellite DNA binding [TAS]
- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- methyl-CpG binding [IDA, NAS]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- satellite DNA binding [TAS]
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Genetic interaction mapping in mammalian cells using CRISPR interference.
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and ... [more]
Quantitative Score
- -1.167588 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line: HEK293 (EFO:0001182)
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Targeted 107 gene chromatin regulation CRISPRi library
- Significance Threshold: S score < -1
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MBD2 KDM1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| KDM1A MBD2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| KDM1A MBD2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3432457 |
Curated By
- BioGRID