KDM1A
Gene Ontology Biological Process
- blood coagulation [TAS]
- histone H3-K4 demethylation [IDA]
- histone H3-K9 demethylation [IDA]
- muscle cell development [ISS]
- negative regulation of DNA binding [IC]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- negative regulation of protein binding [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein demethylation [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- MRF binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- demethylase activity [IMP]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA]
- histone demethylase activity [IDA]
- histone demethylase activity (H3-K4 specific) [IDA]
- histone demethylase activity (H3-K9 specific) [IDA]
- histone demethylase activity (H3-dimethyl-K4 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP]
- oxidoreductase activity [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- transcription factor binding [IDA]
- transcription regulatory region DNA binding [ISS]
- MRF binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- demethylase activity [IMP]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA]
- histone demethylase activity [IDA]
- histone demethylase activity (H3-K4 specific) [IDA]
- histone demethylase activity (H3-K9 specific) [IDA]
- histone demethylase activity (H3-dimethyl-K4 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP]
- oxidoreductase activity [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- transcription factor binding [IDA]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
MBD2
Gene Ontology Biological Process
Gene Ontology Molecular Function- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- methyl-CpG binding [IDA, NAS]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- satellite DNA binding [TAS]
- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- methyl-CpG binding [IDA, NAS]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- satellite DNA binding [TAS]
Gene Ontology Cellular Component
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.712)
- MCF7 cell line (score 0.677)
- MDA231 cell line (score 0.693)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MBD2 KDM1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| KDM1A MBD2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| KDM1A MBD2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -1.1676 | BioGRID | 2537721 |
Curated By
- BioGRID