BAIT
MMP25
MMP-25, MMP20, MMP20A, MMPL1, MT-MMP 6, MT-MMP6, MT6-MMP, MT6MMP, MTMMP6
matrix metallopeptidase 25
GO Process (2)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
PIK3R2
MPPH, MPPH1, P85B, p85, p85-BETA
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
GO Process (24)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- T cell receptor signaling pathway [TAS]
- blood coagulation [TAS]
- cellular glucose homeostasis [ISS]
- cellular response to insulin stimulus [ISS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol 3-kinase signaling [IDA]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol-3-phosphate biosynthetic process [ISS]
- phosphatidylinositol-mediated signaling [TAS]
- phospholipid metabolic process [TAS]
- positive regulation of transcription factor import into nucleus [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- regulation of autophagy [IMP]
- regulation of small GTPase mediated signal transduction [TAS]
- response to endoplasmic reticulum stress [ISS]
- small GTPase mediated signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -5.051 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID