BAIT
MAP2K5
HsT17454, MAPKK5, MEK5, PRKMK5
mitogen-activated protein kinase kinase 5
GO Process (16)
GO Function (2)
GO Component (0)
Gene Ontology Biological Process
- activation of MAPK activity [ISS]
- cellular response to growth factor stimulus [ISS]
- cellular response to laminar fluid shear stress [TAS]
- negative regulation of NF-kappaB transcription factor activity [ISS]
- negative regulation of cell migration involved in sprouting angiogenesis [ISS]
- negative regulation of chemokine (C-X-C motif) ligand 2 production [ISS]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISS]
- negative regulation of heterotypic cell-cell adhesion [ISS]
- negative regulation of interleukin-8 biosynthetic process [ISS]
- negative regulation of response to cytokine stimulus [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of protein metabolic process [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
PIK3CG
PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma
GO Process (25)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- T cell activation [TAS]
- T cell chemotaxis [TAS]
- T cell proliferation [TAS]
- adaptive immune response [TAS]
- blood coagulation [TAS]
- cytokine production [TAS]
- dendritic cell chemotaxis [TAS]
- inflammatory response [TAS]
- innate immune response [TAS]
- mast cell degranulation [TAS]
- natural killer cell chemotaxis [TAS]
- negative regulation of cardiac muscle contraction [TAS]
- neutrophil chemotaxis [TAS]
- neutrophil extravasation [TAS]
- phosphatidylinositol 3-kinase signaling [IDA, TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phospholipid metabolic process [TAS]
- platelet activation [TAS]
- platelet aggregation [TAS]
- positive regulation of MAP kinase activity [IDA]
- positive regulation of protein kinase B signaling [IDA]
- regulation of cell adhesion mediated by integrin [TAS]
- respiratory burst involved in defense response [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -3.216 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID