BAIT
GMPS
guanine monphosphate synthase
GO Process (5)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Homo sapiens
PREY
SPHK1
SPHK
sphingosine kinase 1
GO Process (23)
GO Function (8)
GO Component (3)
Gene Ontology Biological Process
- 'de novo' posttranslational protein folding [TAS]
- calcium-mediated signaling [IDA]
- cellular protein metabolic process [TAS]
- intracellular signal transduction [TAS]
- lipid phosphorylation [IDA]
- negative regulation of apoptotic process [IDA, TAS]
- positive regulation of NF-kappaB import into nucleus [IMP]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of angiogenesis [IDA]
- positive regulation of cell growth [IDA]
- positive regulation of cell migration [IDA]
- positive regulation of fibroblast proliferation [IDA]
- positive regulation of mitotic cell cycle [IDA]
- positive regulation of protein ubiquitination [IDA]
- positive regulation of smooth muscle contraction [IDA]
- protein folding [TAS]
- regulation of tumor necrosis factor-mediated signaling pathway [IDA]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- sphingoid catabolic process [NAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
- sphingosine metabolic process [IDA]
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -3.196 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID