BAIT
LARS2
LEURS, PRLTS4
leucyl-tRNA synthetase 2, mitochondrial
GO Process (3)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PIK3CD
APDS, IMD14, P110DELTA, PI3K, p110D, RP11-558F24.3
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta
GO Process (32)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- B cell activation [TAS]
- B cell chemotaxis [TAS]
- B cell receptor signaling pathway [TAS]
- Fc-epsilon receptor signaling pathway [TAS]
- T cell activation [TAS]
- T cell chemotaxis [TAS]
- T cell differentiation [TAS]
- T cell receptor signaling pathway [TAS]
- adaptive immune response [TAS]
- cytokine production [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- inflammatory response [TAS]
- innate immune response [TAS]
- mast cell chemotaxis [TAS]
- mast cell degranulation [TAS]
- mast cell differentiation [TAS]
- natural killer cell activation [TAS]
- natural killer cell chemotaxis [TAS]
- natural killer cell differentiation [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- neutrophil chemotaxis [TAS]
- neutrophil extravasation [TAS]
- phosphatidylinositol 3-kinase signaling [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol-3-phosphate biosynthetic process [NAS]
- phosphatidylinositol-mediated signaling [TAS]
- phospholipid metabolic process [TAS]
- protein phosphorylation [NAS]
- respiratory burst involved in defense response [TAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -2.919 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID