BAIT

CYP4F12

CYPIVF12, F22329_1, UNQ568/PRO1129
cytochrome P450, family 4, subfamily F, polypeptide 12
Homo sapiens
PREY

MMP25

MMP-25, MMP20, MMP20A, MMPL1, MT-MMP 6, MT-MMP6, MT6-MMP, MT6MMP, MTMMP6
matrix metallopeptidase 25
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.

Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC

Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]

Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]

Quantitative Score

  • -2.915 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507)

Additional Notes

  • CRISPR GI screen
  • Cell Line:K562 (EFO:0002067)
  • Experimental Setup:Timecourse
  • GIST: A-phenotypic negative genetic interaction
  • Library:Drug Target-CDKO CRISPRn library
  • Significance Threshold: q-value<0.05

Curated By

  • BioGRID