BAIT

KSR1

KSR, RSU2
kinase suppressor of ras 1
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

MAP2K2

CFC4, MAPKK2, MEK2, MKK2, PRKMK2
mitogen-activated protein kinase kinase 2
GO Process (31)
GO Function (7)
GO Component (15)
Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.

Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC

Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]

Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]

Quantitative Score

  • -2.859 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507)

Additional Notes

  • CRISPR GI screen
  • Cell Line:K562 (EFO:0002067)
  • Experimental Setup:Timecourse
  • GIST: A-phenotypic negative genetic interaction
  • Library:Drug Target-CDKO CRISPRn library
  • Significance Threshold: q-value<0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MAP2K2 KSR1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID