BAIT
NR5A1
AD4BP, ELP, FTZ1, FTZF1, POF7, SF-1, SF1, SPGF8, SRXY3, RP11-101K10.1
nuclear receptor subfamily 5, group A, member 1
GO Process (8)
GO Function (7)
GO Component (2)
Gene Ontology Biological Process
- cell-cell signaling [TAS]
- gene expression [TAS]
- intracellular receptor signaling pathway [TAS]
- positive regulation of male gonad development [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- primary sex determination [TAS]
- regulation of steroid biosynthetic process [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- enzyme binding [IPI]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [TAS]
- phospholipid binding [IDA]
- protein binding [IPI]
- transcription coactivator activity [TAS]
- DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- enzyme binding [IPI]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [TAS]
- phospholipid binding [IDA]
- protein binding [IPI]
- transcription coactivator activity [TAS]
Gene Ontology Cellular Component
- nucleoplasm [TAS]
- nucleus [IDA]
Homo sapiens
PREY
PRKCZ
PKC-ZETA, PKC2, RP11-181G12.1
protein kinase C, zeta
GO Process (21)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- blood coagulation [TAS]
- establishment of cell polarity [ISS]
- long-term synaptic potentiation [ISS]
- negative regulation of insulin receptor signaling pathway [IMP]
- negative regulation of peptidyl-tyrosine phosphorylation [IMP]
- negative regulation of protein complex assembly [IMP]
- peptidyl-serine phosphorylation [IDA]
- platelet activation [TAS]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of NF-kappaB transcription factor activity [ISS]
- positive regulation of T-helper 2 cell cytokine production [ISS]
- positive regulation of T-helper 2 cell differentiation [ISS]
- positive regulation of excitatory postsynaptic membrane potential [ISS]
- positive regulation of insulin receptor signaling pathway [ISS]
- positive regulation of interleukin-10 secretion [ISS]
- positive regulation of interleukin-13 secretion [ISS]
- positive regulation of interleukin-4 production [ISS]
- positive regulation of interleukin-5 secretion [ISS]
- protein phosphorylation [IDA]
- signal transduction [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -2.7 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library:Drug Target-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID