BAIT
TRAPPC13
C5orf44
trafficking protein particle complex 13
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
TSC2
LAM, PPP1R160, TSC4
tuberous sclerosis 2
GO Process (28)
GO Function (4)
GO Component (7)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- cell cycle arrest [TAS]
- endocytosis [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- heart development [ISS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- insulin-like growth factor receptor signaling pathway [ISS]
- negative regulation of TOR signaling [IBA]
- negative regulation of Wnt signaling pathway [IBA]
- negative regulation of cell proliferation [ISS]
- negative regulation of insulin receptor signaling pathway [IBA]
- negative regulation of phosphatidylinositol 3-kinase signaling [ISS]
- negative regulation of protein kinase B signaling [IBA, ISS]
- negative regulation of protein kinase activity [ISS]
- neural tube closure [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive chemotaxis [ISS]
- positive regulation of Ras GTPase activity [IBA]
- protein import into nucleus [ISS]
- protein kinase B signaling [ISS]
- protein localization [ISS]
- regulation of cell cycle [IBA]
- regulation of endocytosis [ISS]
- regulation of insulin receptor signaling pathway [ISS]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions.
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statistical scoring ... [more]
Nat. Biotechnol. Mar. 20, 2017; 0(); [Pubmed: 28319085]
Quantitative Score
- -1.694 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
Additional Notes
- CRISPR GI screen
- Cell Line:K562 (EFO:0002067)
- Experimental Setup:Toxin Exposure: Ricin at LD50 (first two pulses: 0.25 ng/ml ricin, third pulse: 0.3 ng/ml, fourth pulse: 0.35 ng/ml)
- GIST: A-phenotypic negative genetic interaction
- Library:Ricin-CDKO CRISPRn library
- Significance Threshold: q-value<0.05
Curated By
- BioGRID