Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Structural basis for recruitment of GRIP domain golgin-245 by small GTPase Arl1.

Wu M, Lu L, Hong W, Song H

Recruitment of the GRIP domain golgins to the trans-Golgi network is mediated by Arl1, a member of the ARF/Arl small GTPase family, through interaction between their GRIP domains and Arl1-GTP. The crystal structure of Arl1-GTP in complex with the GRIP domain of golgin-245 shows that Arl1-GTP interacts with the GRIP domain predominantly in a hydrophobic manner, with the switch II ... [more]

Nat. Struct. Mol. Biol. Jan. 01, 2004; 11(1);86-94 [Pubmed: 14718928]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GRIP1 GRIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Liu PY (2006)	Low	-	BioGRID	-
GRIP1 GRIP1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Wu M (2004)	Low	-	BioGRID	-
GRIP1 GRIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Liu PY (2006)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GRIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

androgen receptor binding [NAS]beta-catenin binding [TAS]glucocorticoid receptor binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]receptor signaling complex scaffold activity [NAS]transcription coactivator activity [NAS]

Gene Ontology Cellular Component