BAIT

CEF1

NTC85, L000004270, YMR213W

Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

generation of catalytic spliceosome for second transesterification step [IMP]

mRNA splicing, via spliceosome [IMP]

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]
second spliceosomal transesterification activity [IMP]

Gene Ontology Cellular Component

Prp19 complex [IDA]

U2-type catalytic step 1 spliceosome [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SNR6

U6, L000001961

U6 spliceosomal RNA (U6 snRNA); mediates catalysis of pre-mRNA splicing by the spliceosome; interacts with U4; homology to U6 snRNA, interacts with snr14

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Structures of the Catalytically Activated Yeast Spliceosome Reveal the Mechanism of Branching.

Wan R, Bai R, Yan C, Lei J, Shi Y

Pre-mRNA splicing is executed by the spliceosome. Structural characterization of the catalytically activated complex (Bâˆ—) is pivotal for understanding theÂ branching reaction. In this study, we assembled theÂ Bâˆ— complexes on two different pre-mRNAs from Saccharomyces cerevisiae and determined the cryo-EM structures of four distinct Bâˆ— complexes at overall resolutions of 2.9-3.8Â A. The duplex between U2 small nuclear RNA (snRNA) and the ... [more]

Cell Mar. 06, 2019; (); [Pubmed: 30879786]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CEF1 SNR6	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Bai R (2017)	Low	-	BioGRID	-
CEF1 SNR6	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Liu S (2017)	Low	-	BioGRID	-
SNR6 CEF1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Query CC (2012)	Low	-	BioGRID	644753
SNR6 CEF1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Query CC (2012)	Low	-	BioGRID	644749

Curated By

BioGRID

Interaction Summary