BAIT

SNR6

U6, L000001961

U6 spliceosomal RNA (U6 snRNA); mediates catalysis of pre-mRNA splicing by the spliceosome; interacts with U4; homology to U6 snRNA, interacts with snr14

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

PREY

CEF1

NTC85, L000004270, YMR213W

Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

generation of catalytic spliceosome for second transesterification step [IMP]

mRNA splicing, via spliceosome [IMP]

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]
second spliceosomal transesterification activity [IMP]

Gene Ontology Cellular Component

Prp19 complex [IDA]

U2-type catalytic step 1 spliceosome [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome.

Query CC, Konarska MM

Conformational change within the spliceosome is required between the first and second catalytic steps of pre-mRNA splicing. A prior genetic screen for suppressors of an intron mutant that stalls between the two steps yielded both prp8 and non-prp8 alleles that suppressed second-step splicing defects. We have now identified the strongest non-prp8 suppressors as alleles of the NTC (Prp19 complex) component, ... [more]

Unknown Mar. 08, 2012; 0(0); [Pubmed: 22408182]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)
phenotype: heat sensitivity (APO:0000147)

Additional Notes

U6-U57A (second-step allele) with second-step cef1 alleles resulted in synthetic lethality at 37

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CEF1 SNR6	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Bai R (2017)	Low	-	BioGRID	-
CEF1 SNR6	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Wan R (2019)	Low	-	BioGRID	-
CEF1 SNR6	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Liu S (2017)	Low	-	BioGRID	-
SNR6 CEF1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Query CC (2012)	Low	-	BioGRID	644749

Curated By

BioGRID

Interaction Summary