An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Molecular structure of promoter-bound yeast TFIID.

Kolesnikova O, Ben-Shem A, Luo J, Ranish J, Schultz P, Papai G

Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. Here we present the cryo-electron microscopy structure of promoter-bound yeast TFIID at a resolution better than 5â€‰A, except for a flexible domain. We position the ... [more]

Nat Commun Dec. 07, 2017; 9(1);4666 [Pubmed: 30405110]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TAF7 TAF4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	2	BioGRID	3615172
TAF4 TAF7	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sanders SL (2002)	High	-	BioGRID	-
TAF7 TAF4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sanders SL (2002)	High	-	BioGRID	-
TAF7 TAF4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.169	BioGRID	1947537

Curated By

BioGRID

Interaction Summary

TAF4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]chromatin binding [IDA]

Gene Ontology Cellular Component

TAF7