NRAS
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- Ras protein signal transduction [TAS]
- activation of MAPKK activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MTOR
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- T cell costimulation [TAS]
- TOR signaling [IMP]
- cell growth [IDA, TAS]
- cellular response to hypoxia [ISS]
- cellular response to nutrient levels [ISS]
- double-strand break repair via homologous recombination [IBA]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- growth [NAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- negative regulation of autophagy [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-serine phosphorylation [IMP]
- phosphatidylinositol-mediated signaling [TAS]
- phosphorylation [IDA]
- positive regulation of gene expression [IMP]
- positive regulation of lipid biosynthetic process [IMP]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of transcription from RNA polymerase III promoter [IMP]
- positive regulation of translation [IDA]
- protein autophosphorylation [IDA]
- protein catabolic process [TAS]
- protein phosphorylation [IDA, IMP]
- regulation of actin cytoskeleton organization [IMP]
- response to amino acid [IDA]
- response to nutrient [NAS]
- response to stress [IMP]
- signal transduction [NAS]
Gene Ontology Molecular Function- RNA polymerase III type 1 promoter DNA binding [IDA]
- RNA polymerase III type 2 promoter DNA binding [IDA]
- RNA polymerase III type 3 promoter DNA binding [IDA]
- TFIIIC-class transcription factor binding [IDA]
- kinase activity [IDA, TAS]
- phosphoprotein binding [IPI]
- protein binding [IPI]
- protein dimerization activity [IBA]
- protein serine/threonine kinase activity [IDA, TAS]
- RNA polymerase III type 1 promoter DNA binding [IDA]
- RNA polymerase III type 2 promoter DNA binding [IDA]
- RNA polymerase III type 3 promoter DNA binding [IDA]
- TFIIIC-class transcription factor binding [IDA]
- kinase activity [IDA, TAS]
- phosphoprotein binding [IPI]
- protein binding [IPI]
- protein dimerization activity [IBA]
- protein serine/threonine kinase activity [IDA, TAS]
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
Proximity-dependent biotin labeling (BioID) may identify new targets for cancers driven by difficult-to-drug oncogenes such as Ras. Therefore, BioID was used with wild-type (WT) and oncogenic mutant (MT) H-, K-, and N-Ras, identifying known interactors, including Raf and PI3K, as well as a common set of 130 novel proteins proximal to all Ras isoforms. A CRISPR screen of these proteins for ... [more]
Throughput
- Low Throughput
Additional Notes
- CRISPR GI screen
- Cell Line: MM415, MM485, AsPC1, Caco2, LS174T, T24
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Targeted RAS interactor library
- Significance Threshold: FDR
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NRAS MTOR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NRAS MTOR | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 2548780 | |
| NRAS MTOR | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - | |
| NRAS MTOR | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 2548480 |
Curated By
- BioGRID