Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.

Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, Khavari PA

Proximity-dependent biotin labeling (BioID) may identify new targets for cancers driven by difficult-to-drug oncogenes such as Ras. Therefore, BioID was used with wild-type (WT) and oncogenic mutant (MT) H-, K-, and N-Ras, identifying known interactors, including Raf and PI3K, as well as a common set of 130 novel proteins proximal to all Ras isoforms. A CRISPR screen of these proteins for ... [more]

Mol. Cell Feb. 21, 2019; 73(4);830-844.e12 [Pubmed: 30639242]

Throughput

  • Low Throughput

Additional Notes

  • CRISPR GI screen
  • Cell Line: MM415, MM485, AsPC1, Caco2, LS174T, T24
  • Experimental Setup: Timecourse
  • GIST: A-phenotypic negative genetic interaction
  • Library: Targeted RAS interactor library
  • Significance Threshold: FDR

Curated By

  • BioGRID