Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Cdc37 interacts with the glycine-rich loop of Hsp90 client kinases.

Terasawa K, Yoshimatsu K, Iemura S, Natsume T, Tanaka K, Minami Y

Recently, we identified a client-binding site of Cdc37 that is required for its association with protein kinases. Phage display technology and liquid chromatography-tandem mass spectrometry (which identifies a total of 33 proteins) consistently identify a unique sequence, GXFG, as a Cdc37-interacting motif that occurs in the canonical glycine-rich loop (GXGXXG) of protein kinases, regardless of their dependence on Hsp90 or ... [more]

Mol. Cell. Biol. May. 01, 2006; 26(9);3378-89 [Pubmed: 16611982]

Throughput

  • Low Throughput

Curated By

  • BioGRID