BAIT
CANX
CNX, IP90, P90
calnexin
GO Process (9)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cellular protein metabolic process [TAS]
- clathrin-mediated endocytosis [ISS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein folding [TAS]
- protein secretion [TAS]
- synaptic vesicle endocytosis [ISS]
Gene Ontology Molecular Function
Homo sapiens
PREY
EGLN1
C1orf12, ECYT3, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6, PNAS-118
egl-9 family hypoxia-inducible factor 1
GO Process (10)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
- cellular response to hypoxia [TAS]
- negative regulation of cAMP catabolic process [ISS]
- negative regulation of cyclic-nucleotide phosphodiesterase activity [ISS]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- oxygen homeostasis [IDA]
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline [IDA]
- regulation of angiogenesis [ISS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- response to hypoxia [IDA]
- response to nitric oxide [IDA]
Gene Ontology Molecular Function- enzyme binding [ISS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- peptidyl-proline 4-dioxygenase activity [IDA]
- peptidyl-proline dioxygenase activity [TAS]
- protein binding [IPI]
- enzyme binding [ISS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- peptidyl-proline 4-dioxygenase activity [IDA]
- peptidyl-proline dioxygenase activity [TAS]
- protein binding [IPI]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID