BAIT
CAT
catalase
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- UV protection [IMP]
- hydrogen peroxide catabolic process [IDA]
- negative regulation of apoptotic process [IMP]
- nucleobase-containing small molecule metabolic process [TAS]
- osteoblast differentiation [IDA]
- protein homotetramerization [IDA]
- protein tetramerization [IDA]
- purine nucleobase metabolic process [TAS]
- purine nucleotide catabolic process [TAS]
- response to reactive oxygen species [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
EPHX2
CEH, SEH
epoxide hydrolase 2, cytoplasmic
GO Process (17)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- arachidonic acid metabolic process [TAS]
- cellular calcium ion homeostasis [NAS]
- cholesterol homeostasis [IDA]
- dephosphorylation [IDA]
- drug metabolic process [NAS]
- epoxygenase P450 pathway [TAS]
- inflammatory response [NAS]
- phospholipid dephosphorylation [IDA]
- positive regulation of gene expression [IDA]
- positive regulation of vasodilation [NAS]
- reactive oxygen species metabolic process [NAS]
- regulation of blood pressure [NAS]
- regulation of cholesterol metabolic process [IMP]
- response to toxic substance [NAS]
- small molecule metabolic process [TAS]
- stilbene catabolic process [IDA]
- xenobiotic metabolic process [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Protein-protein interactions govern almost all cellular functions. These complex networks of stable and transient associations can be mapped by affinity purification mass spectrometry (AP-MS) and complementary proximity-based labeling methods such as BioID. To exploit the advantages of both strategies, we here design and optimize an integrated approach combining AP-MS and BioID in a single construct, which we term MAC-tag. We ... [more]
Nat Commun Dec. 22, 2017; 9(1);1188 [Pubmed: 29568061]
Throughput
- High Throughput
Curated By
- BioGRID