BAIT

CDK5RAP2

C48, Cep215, MCPH3, RP11-412P1.1
CDK5 regulatory subunit associated protein 2
Homo sapiens
PREY

BIRC6

APOLLON, BRUCE
baculoviral IAP repeat containing 6
UPS Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions.

Bouguenina H, Salaun D, Mangon A, Muller L, Baudelet E, Camoin L, Tachibana T, Cianferani S, Audebert S, Verdier-Pinard P, Badache A

Control of microtubule dynamics underlies several fundamental processes such as cell polarity, cell division, and cell motility. To gain insights into the mechanisms that control microtubule dynamics during cell motility, we investigated the interactome of the microtubule plus-end-binding protein end-binding 1 (EB1). Via molecular mapping and cross-linking mass spectrometry we identified and characterized a large complex associating a specific isoform ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 12, 2016; 114(50);E10687-E10696 [Pubmed: 29162697]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDK5RAP2 BIRC6
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High54.88BioGRID
2982750

Curated By

  • BioGRID