BAIT
ATP12A
Atp1al1, HKalpha2, cHKA
ATPase, H+/K+ transporting, nongastric, alpha polypeptide
GO Process (6)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
CD63
C75951, ME491, Tspan30
CD63 antigen
GO Process (15)
GO Function (2)
GO Component (13)
Gene Ontology Biological Process
- cell migration [ISO]
- cell-matrix adhesion [ISO]
- cellular protein localization [ISO]
- endosome to melanosome transport [ISO]
- epithelial cell differentiation [ISO]
- negative regulation of epithelial cell migration [ISO]
- pigment cell differentiation [IMP]
- pigment granule maturation [ISO]
- pigmentation [IMP]
- positive regulation of cell adhesion [ISO]
- positive regulation of endocytosis [ISO]
- positive regulation of integrin-mediated signaling pathway [ISO]
- positive regulation of receptor internalization [ISO]
- regulation of rubidium ion transport [ISO]
- regulation of vascular endothelial growth factor signaling pathway [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell surface [ISO]
- cytoplasm [ISO]
- endosome lumen [ISO]
- endosome membrane [ISO]
- extracellular vesicular exosome [ISO]
- intrinsic component of plasma membrane [ISO]
- late endosome [IDA]
- late endosome membrane [ISO]
- lysosomal membrane [ISO]
- lysosome [IDA, ISO]
- multivesicular body, internal vesicle [ISO]
- plasma membrane [ISO]
- protein complex [ISO]
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
CD63 interacts with the carboxy terminus of the colonic H+-K+-ATPase to decrease [corrected] plasma membrane localization and 86Rb+ uptake.
The carboxy terminus (CT) of the colonic H(+)-K(+)-ATPase is required for stable assembly with the beta-subunit, translocation to the plasma membrane, and efficient function of the transporter. To identify protein-protein interactions involved in the localization and function of HKalpha(2), we selected 84 amino acids in the CT of the alpha-subunit of mouse colonic H(+)-K(+)-ATPase (CT-HKalpha(2)) as the bait in a ... [more]
Am. J. Physiol., Cell Physiol. Jun. 01, 2005; 288(6);C1279-86 [Pubmed: 15647390]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CD63 ATP12A | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID