BAIT

VCP

ALS14, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97
valosin containing protein
GO Process (18)
GO Function (8)
GO Component (13)
Homo sapiens
PREY

CNIH4

CNIH-4, HSPC163
cornichon family AMPA receptor auxiliary protein 4
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches.

Zuzow N, Ghosh A, Leonard M, Liao J, Yang B, Bennett EJ

Previous genetic and biochemical studies from Saccharomyces cerevisiae have identified a critical ribosome-associated quality control complex (RQC) that facilitates resolution of stalled ribosomal complexes. While components of the mammalian RQC have been examined in vitro, a systematic characterization of RQC protein interactions in mammalian cells has yet to be described. Here we utilize both proximity-labeling proteomic approaches, BioID and APEX, ... [more]

Mol. Biol. Cell Dec. 15, 2017; 29(10);1258-1269 [Pubmed: 29540532]

Throughput

  • High Throughput

Additional Notes

  • The 'hit' protein was identified as a mass spectrometry-identified VCP-interactor in an APEX2-based proximity labeling experiment but not in a BioID-based proximity labeling experiment or in a FLAG tag immunoprecipitation.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
VCP CNIH4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID