BAIT

LTN1

C21orf10, C21orf98, RNF160, ZNF294, HSPC087
listerin E3 ubiquitin protein ligase 1
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

RPS6KA1

HU-1, MAPKAPK1A, RSK, RSK1, p90Rsk, RP11-492M19.2
ribosomal protein S6 kinase, 90kDa, polypeptide 1
GO Process (26)
GO Function (4)
GO Component (4)

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches.

Zuzow N, Ghosh A, Leonard M, Liao J, Yang B, Bennett EJ

Previous genetic and biochemical studies from Saccharomyces cerevisiae have identified a critical ribosome-associated quality control complex (RQC) that facilitates resolution of stalled ribosomal complexes. While components of the mammalian RQC have been examined in vitro, a systematic characterization of RQC protein interactions in mammalian cells has yet to be described. Here we utilize both proximity-labeling proteomic approaches, BioID and APEX, ... [more]

Mol. Biol. Cell Dec. 15, 2017; 29(10);1258-1269 [Pubmed: 29540532]

Throughput

  • High Throughput

Additional Notes

  • The 'hit' protein was identified by mass spectrometry in either an APEX-based or a BioID-based proximity label experiment.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPS6KA1 LTN1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2615935

Curated By

  • BioGRID