BAIT

GRWD1

CDW4, GRWD, RRB1, WDR28
glutamate-rich WD repeat containing 1
UPS Project
GO Process (0)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

HSP90AA1

EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2
heat shock protein 90kDa alpha (cytosolic), class A member 1
Alzheimer's Disease Project
Arachidonic Acid Pathway Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system.

Watanabe S, Fujiyama H, Takafuji T, Kayama K, Matsumoto M, Nakayama KI, Yoshida K, Sugimoto N, Fujita M

Glutamate-rich WD40 repeat-containing 1 (GRWD1) is a Cdt1-binding protein that promotes mini-chromosome maintenance (MCM) loading through its histone chaperone activity. GRWD1 acts as a tumor-promoting factor by downregulating p53 (also known as TP53) via the RPL11-MDM2-p53 axis. Here, we identified GRWD1-interacting proteins using a proteomics approach and showed that GRWD1 interacts with various proteins involved in transcription, translation, DNA replication ... [more]

J. Cell. Sci. Aug. 03, 2018; 131(15); [Pubmed: 29991511]

Throughput

  • High Throughput

Additional Notes

  • The 'hit' proteins were identified in GRWD1 immunoprecipitations performed in low but not high salt.

Curated By

  • BioGRID