BAIT

HSP90

CaO6.7645, CAWG_05553, CaJ7_0234, IPF4596.2, Contig4-3089_0026, IPF20556.1, C7_02030W_B, orf19.13868, orf19_6515, CaO19.6515, CaO19_6515, orf6.7645, CaO19.13868, CA4959, C7_02030W, orf19.6515, CaJ7.0234, C7_02030W_A
Essential chaperone, regulates several signal transduction pathways and temperature-induced morphogenesis; activated by heat shock, stress; localizes to surface of hyphae, not yeast cells; mediates echinocandin and biofilm azole resistance
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)
PREY

ECM17

orf6.6837, CaO19.11580, CaO6.4171, CaO6.6837, CAWG_04360, Contig4-2950_0017, IPF2102.1, CA4320, CaO19.12932, C2_06170C_B, CaO19.4099, CaO19.5477, IPF13588.2, orf19.11580, IPF23129.1, IPF21658.1, C2_06170C, MET5, orf19.12932, orf19.5477, orf19.4099, orf6.4171, C2_06170C_A
Putative sulfite reductase beta subunit; role in cell wall biogenesis; regulated by Tsa1/Tsa1B in H2O2 stress; Gcn4-regulated; Tbf1-activated; Hap43-repressed; Spider, flow, F-12/CO2 model biofilm induced
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global proteomic analyses define an environmentally contingent Hsp90 interactome and reveal chaperone-dependent regulation of stress granule proteins and the R2TP complex in a fungal pathogen.

O'Meara TR, O'Meara MJ, Polvi EJ, Pourhaghighi MR, Liston SD, Lin ZY, Veri AO, Emili A, Gingras AC, Cowen LE

Hsp90 is a conserved molecular chaperone that assists in the folding and function of diverse cellular regulators, with a profound impact on biology, disease, and evolution. As a central hub of protein interaction networks, Hsp90 engages with hundreds of protein-protein interactions within eukaryotic cells. These interactions include client proteins, which physically interact with Hsp90 and depend on the chaperone for ... [more]

PLoS Biol. Jul. 08, 2019; 17(7);e3000358 [Pubmed: 31283755]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture-MS under basal conditions (Table S1)
  • Used Hsp90 E36A mutant which traps interacting proteins by decreasing Hsp90 ATPase activity

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HSP90 ECM17
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

High-BioGRID
868909

Curated By

  • BioGRID