BAIT

HSP90

CaO6.7645, CAWG_05553, CaJ7_0234, IPF4596.2, Contig4-3089_0026, IPF20556.1, C7_02030W_B, orf19.13868, orf19_6515, CaO19.6515, CaO19_6515, orf6.7645, CaO19.13868, CA4959, C7_02030W, orf19.6515, CaJ7.0234, C7_02030W_A
Essential chaperone, regulates several signal transduction pathways and temperature-induced morphogenesis; activated by heat shock, stress; localizes to surface of hyphae, not yeast cells; mediates echinocandin and biofilm azole resistance
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)
PREY

ECM17

orf6.6837, CaO19.11580, CaO6.4171, CaO6.6837, CAWG_04360, Contig4-2950_0017, IPF2102.1, CA4320, CaO19.12932, C2_06170C_B, CaO19.4099, CaO19.5477, IPF13588.2, orf19.11580, IPF23129.1, IPF21658.1, C2_06170C, MET5, orf19.12932, orf19.5477, orf19.4099, orf6.4171, C2_06170C_A
Putative sulfite reductase beta subunit; role in cell wall biogenesis; regulated by Tsa1/Tsa1B in H2O2 stress; Gcn4-regulated; Tbf1-activated; Hap43-repressed; Spider, flow, F-12/CO2 model biofilm induced
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Mapping the Hsp90 Genetic Interaction Network in Candida albicans Reveals Environmental Contingency and Rewired Circuitry.

Diezmann S, Michaut M, Shapiro RS, Bader GD, Cowen LE

The molecular chaperone Hsp90 regulates the folding of diverse signal transducers in all eukaryotes, profoundly affecting cellular circuitry. In fungi, Hsp90 influences development, drug resistance, and evolution. Hsp90 interacts with ∼10% of the proteome in the model yeast Saccharomyces cerevisiae, while only two interactions have been identified in Candida albicans, the leading fungal pathogen of humans. Utilizing a chemical genomic ... [more]

PLoS Genet. Mar. 01, 2012; 8(3);e1002562 [Pubmed: 22438817]

Throughput

  • High Throughput

Ontology Terms

  • stress resistance (APO:0000080)

Additional Notes

  • This is a chemical genetic screening that kills Hsp90 activity by pharmacological inhibition and looks for growth defect under stress conditions when combined with additional gene deletion.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HSP90 ECM17
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2572896

Curated By

  • BioGRID