BAIT

TP53BP1

53BP1, p202
tumor protein p53 binding protein 1
Homo sapiens
PREY

PPP5C

PP5, PPP5, PPT
protein phosphatase 5, catalytic subunit
Alzheimer's Disease Project
Cell Cycle - Mitotic Spindle Assembly Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Protein phosphatase 5 regulates the function of 53BP1 after neocarzinostatin-induced DNA damage.

Kang Y, Lee JH, Hoan NN, Sohn HM, Chang IY, You HJ

53BP1 (p53-binding protein 1) is a conserved nuclear protein that is phosphorylated in response to DNA damage and rapidly recruited to the site of DNA double strand breaks, demonstrating its role in the early events to DNA damage and repair of damaged DNA. In this study, we used the yeast two-hybrid system to identify proteins that interact with 53BP1. Identification ... [more]

J. Biol. Chem. Apr. 10, 2009; 284(15);9845-53 [Pubmed: 19176521]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TP53BP1 PPP5C
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID