BAIT

KES1

BSR3, LPI3, OSH4, oxysterol-binding protein KES1, L000000894, YPL145C
One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

STE20

mitogen-activated protein kinase kinase kinase kinase STE20, L000002123, YHL007C
Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The oxysterol binding protein Kes1p regulates Golgi apparatus phosphatidylinositol-4-phosphate function.

Fairn GD, Curwin AJ, Stefan CJ, McMaster CR

The Saccharomyces cerevisiae phosphatidylcholine/phosphatidylinositol transfer protein Sec14p is required for Golgi apparatus-derived vesicular transport through coordinate regulation of phospholipid metabolism. Sec14p is normally essential. The essential requirement for SEC14 can be bypassed by inactivation of (i) the CDP-choline pathway for phosphatidylcholine synthesis or (ii) KES1, which encodes an oxysterol binding protein. A unique screen was used to determine genome-wide genetic ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 25, 2007; 104(39);15352-7 [Pubmed: 17881569]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • An SGA screen was used to identify genes specifically required for viability of the query strain which was a sec14 kes1 double mutant.

Curated By

  • BioGRID