An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis.

Lee J, Iwai T, Yokota T, Yamashita M

Sister chromatid cohesion is maintained from DNA replication to metaphase-to-anaphase transition by multisubunit protein complexes called cohesin, which include at least four proteins, SMC1alpha, SMC3, Rad21 and either SA1 or SA2, in mammalian somatic cells. We report here the first evidence of the involvement of Rec8 protein, a mammalian homolog of yeast Rec8p, in meiosis-specific chromosome behavior in mammals. In ... [more]

J. Cell. Sci. Jul. 01, 2003; 116(0);2781-90 [Pubmed: 12759374]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SMC3

Gene Ontology Biological Process

Gene Ontology Molecular Function

dynein binding [IDA]microtubule motor activity [NAS]protein binding [IPI]protein heterodimerization activity [IPI]

Gene Ontology Cellular Component

SYCP3