An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

Telekawa C, Boisvert FM, Bachand F

The RNA exosome is a conserved multi-subunit complex essential for processing and degradation of several types of RNAs. Although many of the functions of the RNA exosome are well established, whether the activity of this complex is regulated remains unclear. Here we performed a proteomic analysis of the RNA exosome complex purified from Schizosaccharomyces pombe and identified 39 post-translational modifications ... [more]

Nucleic Acids Res. Dec. 30, 2017; 46(21);11169-11183 [Pubmed: 30321377]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DIS1 DIS3	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Kinoshita N (1991)	Low	-	BioGRID	737162

Curated By

BioGRID

Interaction Summary

DIS3

Gene Ontology Biological Process

Gene Ontology Molecular Function

3'-5'-exoribonuclease activity [ISO]Ran GTPase binding [IPI]endoribonuclease activity [ISO]protein binding [IPI]ribonuclease activity [IMP]

Gene Ontology Cellular Component

DIS1