An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Ubiquilin 2 modulates ALS/FTD-linked FUS-RNA complex dynamics and stress granule formation.

Alexander EJ, Ghanbari Niaki A, Zhang T, Sarkar J, Liu Y, Nirujogi RS, Pandey A, Myong S, Wang J

The ubiquitin-like protein ubiquilin 2 (UBQLN2) has been genetically and pathologically linked to the neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), but its normal cellular functions are not well understood. In a search for UBQLN2-interacting proteins, we found an enrichment of stress granule (SG) components, including ALS/FTD-linked heterogeneous ribonucleoprotein fused in sarcoma (FUS). Through the use of ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 04, 2017; 115(49);E11485-E11494 [Pubmed: 30442662]

Throughput

High Throughput

Additional Notes

assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HSD17B12 UBQLN2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2731041

Curated By

BioGRID

Interaction Summary

UBQLN2

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

HSD17B12

Gene Ontology Biological Process