FBXL3
Gene Ontology Biological Process
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA, ISO]
- entrainment of circadian clock by photoperiod [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein destabilization [IMP]
- protein ubiquitination [IDA]
- regulation of circadian rhythm [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CRY2
Gene Ontology Biological Process
- circadian regulation of gene expression [IGI, IMP]
- circadian rhythm [IMP]
- entrainment of circadian clock by photoperiod [IMP]
- glucose homeostasis [IGI]
- lipid storage [IGI]
- negative regulation of circadian rhythm [IDA]
- negative regulation of glucocorticoid receptor signaling pathway [IGI]
- negative regulation of glucocorticoid secretion [IGI]
- negative regulation of phosphoprotein phosphatase activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [IDA, IGI, ISO]
- protein import into nucleus [IPI]
- regulation of circadian rhythm [IMP]
- response to insulin [IGI]
Gene Ontology Molecular Function- DNA binding [ISO]
- FAD binding [IDA]
- damaged DNA binding [ISO]
- kinase binding [IPI]
- nuclear hormone receptor binding [IPI]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- single-stranded DNA binding [ISO]
- transcription factor binding transcription factor activity [ISO]
- transcription regulatory region sequence-specific DNA binding [IDA]
- ubiquitin binding [ISO]
- DNA binding [ISO]
- FAD binding [IDA]
- damaged DNA binding [ISO]
- kinase binding [IPI]
- nuclear hormone receptor binding [IPI]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- single-stranded DNA binding [ISO]
- transcription factor binding transcription factor activity [ISO]
- transcription regulatory region sequence-specific DNA binding [IDA]
- ubiquitin binding [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The circadian E3 ligase complex SCFFBXL3+CRY targets TLK2.
We recently demonstrated that the circadian clock component CRY2 is an essential cofactor in the SCFFBXL3-mediated ubiquitination of c-MYC. Because our demonstration that CRY2 recruits phosphorylated substrates to SCFFBXL3 was unexpected, we investigated the scope of this role by searching for additional substrates of FBXL3 that require CRY1 or CRY2 as cofactors. Here, we describe an affinity purification mass spectrometry ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FBXL3 CRY2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| FBXL3 CRY2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID