YY1
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISS]
- four-way junction DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [TAS]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
- DNA binding [IDA]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISS]
- four-way junction DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [TAS]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
Gene Ontology Cellular Component
YY1
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISS]
- four-way junction DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [TAS]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
- DNA binding [IDA]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISS]
- four-way junction DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [TAS]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Structure of Yin Yang 1 oligomers that cooperate with RuvBL1-RuvBL2 ATPases.
Yin Yang 1 (YY1) is a transcription factor regulating proliferation and differentiation and is involved in cancer development. Oligomers of recombinant YY1 have been observed before, but their structure and DNA binding properties are not well understood. Here we find that YY1 assembles several homo-oligomeric species built from the association of a bell-shaped dimer, a process we characterized by electron ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| YY1 YY1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| YY1 YY1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID