BAIT
GBF1
ARF1GEF
golgi brefeldin A resistant guanine nucleotide exchange factor 1
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PLA2G4A
PLA2G4, cPLA2-alpha
phospholipase A2, group IVA (cytosolic, calcium-dependent)
GO Process (15)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- arachidonic acid metabolic process [TAS]
- blood coagulation [TAS]
- cardiolipin acyl-chain remodeling [TAS]
- glycerophospholipid biosynthetic process [TAS]
- icosanoid metabolic process [NAS]
- phosphatidic acid biosynthetic process [TAS]
- phosphatidylcholine acyl-chain remodeling [TAS]
- phosphatidylethanolamine acyl-chain remodeling [TAS]
- phosphatidylglycerol acyl-chain remodeling [TAS]
- phosphatidylinositol acyl-chain remodeling [TAS]
- phosphatidylserine acyl-chain remodeling [TAS]
- phospholipid metabolic process [TAS]
- platelet activating factor biosynthetic process [NAS]
- platelet activation [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
BioID Performed on Golgi Enriched Fractions Identify C10orf76 as a GBF1 Binding Protein Essential for Golgi Maintenance and Secretion.
The Golgi-specific Brefeldin-A resistance factor 1 (GBF1) is the only large GEF that regulates Arf activation at the cis-Golgi and is actively recruited to membranes on an increase in Arf-GDP. Recent studies have revealed that GBF1 recruitment requires one or more heat-labile and protease-sensitive protein factor(s) (Quilty et al., 2018, J. Cell Science, 132). Proximity-dependent biotinylation (BioID) and mass spectrometry ... [more]
Mol. Cell Proteomics Nov. 01, 2019; 18(11);2285-2297 [Pubmed: 31519766]
Throughput
- High Throughput
Additional Notes
- BioID system
- Proteins with two or more unique peptides and having a Bayesian False Discovery Rate of 1% (BFDR less than or equal to 0.01) are high-confidence proximity interactors.
Curated By
- BioGRID