BAIT
RFFL
CARP-2, CARP2, FRING, RIFIFYLIN, RNF189, RNF34L
ring finger and FYVE-like domain containing E3 ubiquitin protein ligase
GO Process (10)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis [IMP]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- negative regulation of signal transduction by p53 class mediator [IMP]
- negative regulation of tumor necrosis factor-mediated signaling pathway [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein K48-linked ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of TOR signaling [IC]
- regulation of fibroblast migration [ISS]
- ubiquitin-dependent protein catabolic process [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB8A
MEL, RAB8
RAB8A, member RAS oncogene family
GO Process (15)
GO Function (6)
GO Component (16)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- GTP catabolic process [IBA, IDA]
- Golgi vesicle fusion to target membrane [IDA]
- Rab protein signal transduction [IBA]
- axonogenesis [ISS]
- cellular response to insulin stimulus [IBA, ISS]
- cilium assembly [IDA, IMP]
- intracellular protein transport [IBA]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- protein localization to plasma membrane [IBA, ISS]
- protein secretion [IBA]
- regulation of exocytosis [IBA]
- synaptic vesicle exocytosis [IBA]
- vesicle docking involved in exocytosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [TAS]
- centrosome [IDA]
- cilium [IDA]
- cytoplasmic vesicle membrane [TAS]
- extracellular vesicular exosome [IDA]
- nonmotile primary cilium [IDA]
- nucleolus [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
- phagocytic vesicle [IDA]
- plasma membrane [IBA, IDA]
- primary cilium [IDA]
- recycling endosome membrane [IDA]
- secretory granule membrane [IBA]
- synaptic vesicle [IBA]
- trans-Golgi network transport vesicle [IBA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors.
Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ubiquitylation of endocytic recycling regulators. An RFFL dominant-negative (DN) mutant induced clustering of endocytic ... [more]
J. Cell. Sci. Dec. 07, 2018; 132(3); [Pubmed: 30659120]
Throughput
- High Throughput
Additional Notes
- BioID
Curated By
- BioGRID