CCDC88A
Gene Ontology Biological Process
- TOR signaling [ISS]
- activation of protein kinase B activity [ISS]
- cell migration [IMP]
- lamellipodium assembly [IMP]
- membrane organization [IDA]
- regulation of DNA replication [ISS]
- regulation of actin cytoskeleton organization [IMP]
- regulation of cell proliferation [ISS]
- regulation of neuron projection development [ISS]
- regulation of protein phosphorylation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DYNC1H1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The human cytoplasmic dynein interactome reveals novel activators of motility.
In human cells, cytoplasmic dynein-1 is essential for long-distance transport of many cargos, including organelles, RNAs, proteins, and viruses, towards microtubule minus ends. To understand how a single motor achieves cargo specificity, we identified the human dynein interactome by attaching a promiscuous biotin ligase ('BioID') to seven components of the dynein machinery, including a subunit of the essential cofactor dynactin. ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID