BAIT

GJB1

CMTX, CMTX1, CX32
gap junction protein, beta 1, 32kDa
GO Process (5)
GO Function (0)
GO Component (1)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration.

Coelho JPL, Stahl M, Bloemeke N, Meighen-Berger K, Alvira CP, Zhang ZR, Sieber SA, Feige MJ

A fundamental step in membrane protein biogenesis is their integration into the lipid bilayer with a defined orientation of each transmembrane segment. Despite this, it remains unclear how cells detect and handle failures in this process. Here we show that single point mutations in the membrane protein connexin 32 (Cx32), which cause Charcot-Marie-Tooth disease, can cause failures in membrane integration. ... [more]

Nat Commun Dec. 08, 2018; 10(1);672 [Pubmed: 30737405]

Throughput

  • Low Throughput

Additional Notes

  • Source of GJB1 not clear

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GJB1 CANX
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2623887

Curated By

  • BioGRID